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Mouse Monoclonal AntibodiesCat. No. GAM920920

CD274 mouse monoclonal antibody,clone OTI13G7

AliasesCD274
ReactivityHuman
ApplicationsELISA · FC · IF
Host / ClonalityMouse / Monoclonal
Lead timeIn stock · ships in 48h
$169/ 50μlIn stock 199 · Ready to ship
50μl$169
100μl$299

Applications & Recommended Dilution

Applications & DilutionRecommended Dilution
ELISA
Recommended dilutionNo data
Reactivity
Human
Assay
ELISA
FC
Recommended dilutionNo data
Reactivity
Human
Assay
FC
IF
Recommended dilutionNo data
Reactivity
Human
Assay
IF

Product Images

Product ImagesImages
CD274 mouse monoclonal antibody,clone OTI13G7 图 1
Image 1 / 7No text caption for this image
CD274 mouse monoclonal antibody,clone OTI13G7 图 2
Image 2 / 7Immunocytochemistry staining of CD274 stable expression cells using anti-CD274 mouse monoclonal antibody (GAM920920) (Left). The right is negative control (1:5000).
CD274 mouse monoclonal antibody,clone OTI13G7 图 3
Image 3 / 7Flow cytometric Analysis of HCC78 cells, using anti-PDL1 antibody (GAM920920), (Red), compared to isotype control, (green), and negative control (PBS), (Blue) (1:100).
CD274 mouse monoclonal antibody,clone OTI13G7 图 4
Image 4 / 7Flow cytometric Analysis of stable expression CD274 cells using anti-CD274 antibody (GAM920920) (Red) compared to a nonspecific negative control antibody (Blue). The left is 293T as negative control (1:100).
CD274 mouse monoclonal antibody,clone OTI13G7 图 5
Image 5 / 7Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-PDL1 antibody (GAM920920) (1:100).
CD274 mouse monoclonal antibody,clone OTI13G7 图 6
Image 6 / 7Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right) using anti-PDL1 antibody (GAM920920). Cells incubated with a non-specific antibody (Left) were used as isotype control (1:100).
CD274 mouse monoclonal antibody,clone OTI13G7 图 7
Image 7 / 7Detection of PDL1 neutralizing antibody using MACS column. GFP+/PDL1+ 293T cells (co-transfected with PDL1 and GFP plasmid (PS10010) were incubated with either PDL1 antibody GAM920920 (red), non-specific antibody (green), isotype control (blue) or PBS (black) and then mixed with PD1+ 293T cells linked with magnetic-beads. The mixed cells were pulled down using MACS column (Miltenyi Biotec) and analysed by Flow Cytometry. GFP+/PDL1+ cells would not be collected if PD1/PDL1 interaction is neutralized by the tested antibody (1:50).

Product Specifications

SpecificationsProduct Specifications
Core Attributes CORE ATTRIBUTES
Species Reactivity
Human
Applications
ELISAFCIF
Clone name
OTI13G7
Calculated molecular weight
31 kDa
Immunogen
Human recombinant protein fragment corresponding to amino acids 19-239 of human PD-L1/CD274(NP_054862) produced in HEK293 cells.
Isotype
IgG1
Host species
Mouse
Clonality
Monoclonal
Purification method
Affinity Purification
Uniprot-A
Q9NZQ7
Product Properties PRODUCT PROPERTIES
Shipping
They are shipped out with dry ice/blue ice unless customers require otherwise
Storage Condition
Store at -20°C PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.Stable for 12 months from date of receipt.
Formulation
Liquid
Storage Buffer
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Shipping

Storage & ShippingHandling

01Shipping

They are shipped out with dry ice/blue ice unless customers require otherwise

02Storage Condition

Store at -20°C PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.Stable for 12 months from date of receipt.

03Formulation

Liquid

04Storage Buffer

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

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