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Mouse Monoclonal AntibodiesCat. No. GAM611400

VEGFA (VEGF) mouse monoclonal antibody, clone OTI4E3 (formerly 4E3)

Name: VEGFA (VEGF) mouse monoclonal antibody, clone OTI4E3 (formerly 4E3)
SKU: GAM611400
Price: 169 CNY
Availability: InStock

AliasesVEGFA

ReactivityHuman

ApplicationsIF · IHC · WB

Host / ClonalityMouse / Monoclonal

Lead timeIn stock · ships in 48h

$169/ 50μlIn stock 200 · Ready to ship

50μl$169

100μl$299

Applications & Recommended Dilution

Applications & DilutionRecommended Dilution

Recommended dilutionNo data

Reactivity: Human
Assay: IF

IHC

Recommended dilution1:50

Reactivity: Human
Assay: IHC

Recommended dilutionNo data

Reactivity: Human
Assay: WB

Product Images

Product ImagesImages

VEGFA (VEGF) mouse monoclonal antibody, clone OTI4E3 (formerly 4E3) 图 1 — Image 1 / 6Anti-VEGF mouse monoclonal antibody (GAM611400) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VEGF

VEGFA (VEGF) mouse monoclonal antibody, clone OTI4E3 (formerly 4E3) 图 2 — Image 2 / 6Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-VEGF mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

VEGFA (VEGF) mouse monoclonal antibody, clone OTI4E3 (formerly 4E3) 图 3 — Image 3 / 6Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VEGF mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

VEGFA (VEGF) mouse monoclonal antibody, clone OTI4E3 (formerly 4E3) 图 4 — Image 4 / 6Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-VEGF mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

VEGFA (VEGF) mouse monoclonal antibody, clone OTI4E3 (formerly 4E3) 图 5 — Image 5 / 6Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-VEGF monoclonal antibody (1:200).

VEGFA (VEGF) mouse monoclonal antibody, clone OTI4E3 (formerly 4E3) 图 6 — Image 6 / 6Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VEGF mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Product Specifications

SpecificationsProduct Specifications

Core Attributes CORE ATTRIBUTES

Species Reactivity: Human

Applications: IFIHCWB

Clone name: OTI4E3

Calculated molecular weight: 23.8 kDa

Immunogen: Human recombinant protein fragment corresponding to amino acids 27-233 of human VEGFA (NP_003367) produced in E.coli.

Isotype: IgG1

Host species: Mouse

Clonality: Monoclonal

Purification method: Affinity Purification

Uniprot-A: P15692

Recommended Dilution RECOMMENDED DILUTION

IHC: 1:50

Product Properties PRODUCT PROPERTIES

Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise

Storage Condition: Store at -20°C PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.Stable for 12 months from date of receipt.

Formulation: Liquid

Storage Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

VEGF is a member of the PDGF/VEGF growth factor family that is often found as a disulfide linked homodimer. It is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in VEGF gene have been associated with proliferative and nonproliferative diabetic retinopathy.