Products

Custom Services

Technical Support

News Center

Promotions

About Us

Products

Custom Services

Technical Support

News Center

Promotions

About Us

Mouse Monoclonal AntibodiesCat. No. GAM612986

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11)

Name: RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11)
SKU: GAM612986
Price: 169 CNY
Availability: InStock

AliasesRibonuclease Inhibitor

ReactivityDog / Human / Monkey / Rat

ApplicationsFC · IHC · WB

Host / ClonalityMouse / Monoclonal

Lead timeIn stock · ships in 48h

$169/ 50μlIn stock 200 · Ready to ship

50μl$169

100μl$299

Applications & Recommended Dilution

Applications & DilutionRecommended Dilution

Recommended dilutionNo data

Reactivity: Dog / Human / Monkey / Rat
Assay: FC

IHC

Recommended dilutionNo data

Reactivity: Dog / Human / Monkey / Rat
Assay: IHC

Recommended dilution1:500~2000

Reactivity: Dog / Human / Monkey / Rat
Assay: WB

Product Images

Product ImagesImages

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 图 1 — Image 1 / 13Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (GAM612986). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 图 2 — Image 2 / 13Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RNH1 mouse monoclonal antibody. (GAM612986). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 图 3 — Image 3 / 13Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (GAM612986). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 图 4 — Image 4 / 13Flow cytometric Analysis of Hela cells, using anti-RNH1 antibody (GAM612986), (Red), compared to a nonspecific negative control antibody (Blue).

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 图 5 — Image 5 / 13Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RNH1 mouse monoclonal antibody. (GAM612986). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 图 6 — Image 6 / 13Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (GAM612986). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 图 7 — Image 7 / 13Simple Western™ analysis of endogenous protein RNH1 from HELA lysates (0.5 mg/mL) using RNH1 Mouse Monoclonal Antibody #GAM612986. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 图 8 — Image 8 / 13Western blot analysis of extracts (30ug per lane) from 4 different cell lines lysates by using anti-RNH1 antibody(GAM612986,1:1000@1mg/ml).

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 图 9 — Image 9 / 13Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (GAM612986). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 图 10 — Image 10 / 13Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (GAM612986). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 图 11 — Image 11 / 13Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RNH1 mouse monoclonal antibody. (GAM612986). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 图 12 — Image 12 / 13Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (GAM612986). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 图 13 — Image 13 / 13Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RNH1 mouse monoclonal antibody. (GAM612986). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Product Specifications

SpecificationsProduct Specifications

Core Attributes CORE ATTRIBUTES

Species Reactivity: DogHumanMonkeyRat

Applications: FCIHCWB

Clone name: OTI3F11

Calculated molecular weight: 49.8 kDa

Immunogen: Full length human recombinant protein of human RNH1 (NP_002930) produced in HEK293T cell.

Isotype: IgG1

Host species: Mouse

Clonality: Monoclonal

Purification method: Affinity Purification

Uniprot-A: P13489

Recommended Dilution RECOMMENDED DILUTION

WB: 1:500~2000

Product Properties PRODUCT PROPERTIES

Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise

Storage Condition: Store at -20°C PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.Stable for 12 months from date of receipt.

Formulation: Liquid

Storage Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo. [supplied by OMIM]