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Mouse Monoclonal AntibodiesCat. No. GAM613659

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)

Name: POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)
SKU: GAM613659
Price: 169 CNY
Availability: InStock

AliasesPOLR2E

ReactivityDog / Human / Monkey / Mouse / Rat

ApplicationsFC · IF · IHC · WB

Host / ClonalityMouse / Monoclonal

Lead timeIn stock · ships in 48h

$169/ 50μlIn stock 200 · Ready to ship

50μl$169

100μl$299

Applications & Recommended Dilution

Applications & DilutionRecommended Dilution

Recommended dilutionNo data

Reactivity: Dog / Human / Monkey / Mouse / Rat
Assay: FC

Recommended dilutionNo data

Reactivity: Dog / Human / Monkey / Mouse / Rat
Assay: IF

IHC

Recommended dilution1:150

Reactivity: Dog / Human / Monkey / Mouse / Rat
Assay: IHC

Recommended dilution1:500~2000

Reactivity: Dog / Human / Monkey / Mouse / Rat
Assay: WB

Product Images

Product ImagesImages

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 图 1 — Image 1 / 13No text caption for this image

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 图 2 — Image 2 / 13Anti-POLR2E mouse monoclonal antibody (GAM613659) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2E

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 图 3 — Image 3 / 13Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POLR2E mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 图 4 — Image 4 / 13No text caption for this image

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 图 5 — Image 5 / 13Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR2E mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 图 6 — Image 6 / 13Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POLR2E mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 图 7 — Image 7 / 13Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR2E mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 图 8 — Image 8 / 13Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR2E mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 图 9 — Image 9 / 13Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-POLR2E mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 图 10 — Image 10 / 13Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POLR2E mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 图 11 — Image 11 / 13Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POLR2E mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 图 12 — Image 12 / 13Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.

POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5) 图 13 — Image 13 / 13Flow cytometric Analysis of Jurkat cells, using anti-POLR2E antibody (GAM613659), (Red), compared to a nonspecific negative control antibody, (Blue).

Product Specifications

SpecificationsProduct Specifications

Core Attributes CORE ATTRIBUTES

Species Reactivity: DogHumanMonkeyMouseRat

Applications: FCIFIHCWB

Clone name: OTI3B5

Calculated molecular weight: 24.4 kDa

Immunogen: Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.

Isotype: IgG2a

Host species: Mouse

Clonality: Monoclonal

Purification method: Affinity Purification

Uniprot-A: P19388

Recommended Dilution RECOMMENDED DILUTION

WB: 1:500~2000

IHC: 1:150

Product Properties PRODUCT PROPERTIES

Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise

Storage Condition: Store at -20°C PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.Stable for 12 months from date of receipt.

Formulation: Liquid

Storage Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]