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Mouse Monoclonal AntibodiesCat. No. GAM614026

CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5)

Name: CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5)
SKU: GAM614026
Price: 169 CNY
Availability: InStock

AliasesCDK2

ReactivityHuman / Mouse / Rat

ApplicationsFC · IF · WB

Host / ClonalityMouse / Monoclonal

Lead timeIn stock · ships in 48h

$169/ 50μlIn stock 200 · Ready to ship

50μl$169

100μl$299

Applications & Recommended Dilution

Applications & DilutionRecommended Dilution

Recommended dilutionNo data

Reactivity: Human / Mouse / Rat
Assay: FC

Recommended dilutionNo data

Reactivity: Human / Mouse / Rat
Assay: IF

Recommended dilution1:2000

Reactivity: Human / Mouse / Rat
Assay: WB

Product Images

Product ImagesImages

CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) 图 1 — Image 1 / 12No text caption for this image

CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) 图 2 — Image 2 / 12Anti-CDK2 mouse monoclonal antibody (GAM614026) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2 (1:100).

CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) 图 3 — Image 3 / 12No text caption for this image

CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) 图 4 — Image 4 / 12Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDK2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) 图 5 — Image 5 / 12Flow cytometric Analysis of Hela cells, using anti-CDK2 antibody (GAM614026), (Red), compared to a nonspecific negative control antibody, (Blue) (1:100).

CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) 图 6 — Image 6 / 12Immunofluorescent staining of HeLa cells using anti-CDK2 mouse monoclonal antibody (GAM614026) (1:100).

CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) 图 7 — Image 7 / 12Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CDK2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) 图 8 — Image 8 / 12Western blot analysis of extracts (35ug) from 14 cell lines by using anti-CDK2 monoclonal antibody (1:500).

CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) 图 9 — Image 9 / 12Flow cytometric Analysis of Jurkat cells, using anti-CDK2 antibody (GAM614026), (Red), compared to a nonspecific negative control antibody, (Blue) (1:100).

CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) 图 10 — Image 10 / 12Simple Western™ analysis of endogenous protein CDK2 from HELA lysates (0.2 mg/mL) using CDK2 Mouse Monoclonal Antibody #GAM614026. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) 图 11 — Image 11 / 12Immunohistochemical staining of paraffin-embedded lung cancer using CDK2 antibody TA502915clone OTI2A5 mouse monoclonal antibody. Protocol used HIER TEE pH9.0 (cat# B21-100)and anti-CDK2 at 1:100 dilution. Detection was done with Polink1 Broad Mouse and Rabbit C/N D11-18 with DAB Kit. Image 40x magnification.

CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5) 图 12 — Image 12 / 12Immunohistochemical staining of paraffin-embedded placenta using CDK2 antibody TA502915 clone OTI2A5 mouse monoclonal antibody. Protocol used HIER TEE pH9.0 (cat# B21-100)and anti-CDK2 at 1:100 dilution. Detection was done with Polink1 Broad Mouse and Rabbit C/N D11-18 with DAB Kit. Image 40x magnification.

Product Specifications

SpecificationsProduct Specifications

Core Attributes CORE ATTRIBUTES

Species Reactivity: HumanMouseRat

Applications: FCIFWB

Clone name: OTI2A5

Calculated molecular weight: 33.7 kDa

Immunogen: Full length human recombinant protein of human CDK2 (NP_001789) produced in HEK293T cell.

Isotype: IgG2a

Host species: Mouse

Clonality: Monoclonal

Purification method: Affinity Purification

Uniprot-A: P24941

Recommended Dilution RECOMMENDED DILUTION

WB: 1:2000

IHC: 1:150

Product Properties PRODUCT PROPERTIES

Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise

Storage Condition: Store at -20°C PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.Stable for 12 months from date of receipt.

Formulation: Liquid

Storage Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq]