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Mouse Monoclonal AntibodiesCat. No. GAM614064

FGF21 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4)

Name: FGF21 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4)
SKU: GAM614064
Price: 169 CNY
Availability: InStock

AliasesFGF21

ReactivityHuman

ApplicationsFC · IF · IHC · WB

Host / ClonalityMouse / Monoclonal

Lead timeIn stock · ships in 48h

$169/ 50μlIn stock 200 · Ready to ship

50μl$169

100μl$299

Applications & Recommended Dilution

Applications & DilutionRecommended Dilution

Recommended dilutionNo data

Reactivity: Human
Assay: FC

Recommended dilutionNo data

Reactivity: Human
Assay: IF

IHC

Recommended dilution1:150

Reactivity: Human
Assay: IHC

Recommended dilutionNo data

Reactivity: Human
Assay: WB

Product Images

Product ImagesImages

FGF21 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4) 图 1 — Image 1 / 9No text caption for this image

FGF21 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4) 图 2 — Image 2 / 9Western blot analysis of extracts (10ug) from Hela cell line by using anti-FGF21 monoclonal antibody at 1:200.

FGF21 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4) 图 3 — Image 3 / 9No text caption for this image

FGF21 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4) 图 4 — Image 4 / 9Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FGF21 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

FGF21 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4) 图 5 — Image 5 / 9Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FGF21 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

FGF21 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4) 图 6 — Image 6 / 9Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-FGF21 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

FGF21 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4) 图 7 — Image 7 / 9Western blot analysis of extracts (10ug) from 1 cell line by using anti-FGF21 monoclonal antibody at 1:200.

FGF21 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4) 图 8 — Image 8 / 9Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FGF21 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

FGF21 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4) 图 9 — Image 9 / 9Recombinant Hu FGF21 protein 100ng/mL was coated on plate and incubated at 4°C for overnight. After blocking with 1%BSA, 2x serially diluted primary antibody GAM614064 (0.78 to 50ng/mL) and 10000x diluted HRP conjugated goat anti-mouse IgG were used for detection. Signal/Background ratio=71, EC50=3.375 ng/ml

Product Specifications

SpecificationsProduct Specifications

Core Attributes CORE ATTRIBUTES

Species Reactivity: Human

Applications: FCIFIHCWB

Clone name: OTI1H4

Calculated molecular weight: 19.4 kDa

Immunogen: Full length human recombinant protein of human FGF21(NP_061986) produced in HEK293 cell.

Isotype: IgG1

Host species: Mouse

Clonality: Monoclonal

Purification method: Affinity Purification

Uniprot-A: Q9NSA1

Recommended Dilution RECOMMENDED DILUTION

IHC: 1:150

Product Properties PRODUCT PROPERTIES

Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise

Storage Condition: Store at -20°C PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.Stable for 12 months from date of receipt.

Formulation: Liquid

Storage Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq]