Products

Custom Services

Technical Support

News Center

Promotions

About Us

Products

Custom Services

Technical Support

News Center

Promotions

About Us

Mouse Monoclonal AntibodiesCat. No. GAM614272

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12)

Name: ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12)
SKU: GAM614272
Price: 169 CNY
Availability: InStock

AliasesApg3

ReactivityHuman / Mouse / Rat

ApplicationsFC · IF · IHC · WB

Host / ClonalityMouse / Monoclonal

Lead timeIn stock · ships in 48h

$169/ 50μlIn stock 200 · Ready to ship

50μl$169

100μl$299

Applications & Recommended Dilution

Applications & DilutionRecommended Dilution

Recommended dilutionNo data

Reactivity: Human / Mouse / Rat
Assay: FC

Recommended dilutionNo data

Reactivity: Human / Mouse / Rat
Assay: IF

IHC

Recommended dilution1:150

Reactivity: Human / Mouse / Rat
Assay: IHC

Recommended dilution1:2000

Reactivity: Human / Mouse / Rat
Assay: WB

Product Images

Product ImagesImages

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 1 — Image 1 / 15No text caption for this image

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 2 — Image 2 / 15Anti-ATG3 mouse monoclonal antibody (GAM614272) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 3 — Image 3 / 15No text caption for this image

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 4 — Image 4 / 15Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATG3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 5 — Image 5 / 15Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ATG3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 6 — Image 6 / 15Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ATG3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 7 — Image 7 / 15Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ATG3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 8 — Image 8 / 15Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 9 — Image 9 / 15Immunofluorescent staining of HeLa cells using anti-ATG3 mouse monoclonal antibody (TA503161).

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 10 — Image 10 / 15Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ATG3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 11 — Image 11 / 15Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ATG3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 12 — Image 12 / 15Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ATG3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 13 — Image 13 / 15Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATG3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 14 — Image 14 / 15Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ATG3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12) 图 15 — Image 15 / 15Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ATG3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Product Specifications

SpecificationsProduct Specifications

Core Attributes CORE ATTRIBUTES

Species Reactivity: HumanMouseRat

Applications: FCIFIHCWB

Clone name: OTI2C12

Calculated molecular weight: 35.7 kDa

Immunogen: Full length human recombinant protein of human ATG3(NP_057393) produced in HEK293 cell.

Isotype: IgG1

Host species: Mouse

Clonality: Monoclonal

Purification method: Affinity Purification

Uniprot-A: Q9NT62

Recommended Dilution RECOMMENDED DILUTION

WB: 1:2000

IHC: 1:150

Product Properties PRODUCT PROPERTIES

Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise

Storage Condition: Store at -20°C PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.Stable for 12 months from date of receipt.

Formulation: Liquid

Storage Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]). [supplied by OMIM]