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Mouse Monoclonal AntibodiesCat. No. GAM615880

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)

Name: PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)
SKU: GAM615880
Price: 169 CNY
Availability: InStock

AliasesPhosphoribosyl pyrophosphate amidotransferase

ReactivityDog / Human / Monkey / Mouse / Rat

ApplicationsIHC · WB

Host / ClonalityMouse / Monoclonal

Lead timeIn stock · ships in 48h

$169/ 50μlIn stock 200 · Ready to ship

50μl$169

100μl$299

Applications & Recommended Dilution

Applications & DilutionRecommended Dilution

IHC

Recommended dilution1:150

Reactivity: Dog / Human / Monkey / Mouse / Rat
Assay: IHC

Recommended dilution1:500~2000

Reactivity: Dog / Human / Monkey / Mouse / Rat
Assay: WB

Product Images

Product ImagesImages

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 1 — Image 1 / 16No text caption for this image

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 2 — Image 2 / 16Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PPAT mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 3 — Image 3 / 16Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PPAT mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 4 — Image 4 / 16Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PPAT mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 5 — Image 5 / 16Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PPAT mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 6 — Image 6 / 16Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PPAT mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 7 — Image 7 / 16Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PPAT mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 8 — Image 8 / 16Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PPAT mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 9 — Image 9 / 16Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PPAT mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 10 — Image 10 / 16Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PPAT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 11 — Image 11 / 16Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-PPAT monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 12 — Image 12 / 16Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PPAT mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 13 — Image 13 / 16Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PPAT mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 14 — Image 14 / 16Western blot analysis of extracts (10ug) from a mouse cell line by using anti-PPAT monoclonal antibody (1:200).

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 15 — Image 15 / 16Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PPAT mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 图 16 — Image 16 / 16Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PPAT mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Product Specifications

SpecificationsProduct Specifications

Core Attributes CORE ATTRIBUTES

Species Reactivity: DogHumanMonkeyMouseRat

Applications: IHCWB

Clone name: OTI1B8

Calculated molecular weight: 57.2 kDa

Immunogen: Human recombinant protein fragment corresponding to amino acids 42-278 of human PPAT(NP_002694) produced in E.coli.

Isotype: IgG1

Host species: Mouse

Clonality: Monoclonal

Purification method: Affinity Purification

Uniprot-A: Q06203

Recommended Dilution RECOMMENDED DILUTION

WB: 1:500~2000

IHC: 1:150

Product Properties PRODUCT PROPERTIES

Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise

Storage Condition: Store at -20°C PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.Stable for 12 months from date of receipt.

Formulation: Liquid

Storage Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.