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Recombinant Protein Expression Service

From mg to 50L Fermenter Scale industrial protein

The Tenzobio recombinant protein expression platform, supplied exclusively through VONE BIO, integrates four expression systems (prokaryotic, yeast, insect, mammalian). Single-batch delivery up to 50L fermenter scale, high-purity protein for structural biology, antibody drug development and diagnostic materials.

Max Fermenter Scale50L
Protein Purity>90%
Endotoxin Level<1EU/mg
Success Rate90%

Four Expression Systems, Every Protein Covered

From prokaryotic to mammalian cells, from mg to gram scale, we find the optimal expression strategy for each protein.

01

Prokaryotic System (E.coli)

Cost-effective for enzymes, toxins, domains. Max yield up to 500mg/L.

Fast DeliveryHigh YieldCost-effective
02

Yeast System (Pichia)

Combines eukaryotic modification with yield, suitable for enzymes, cytokines (100-500mg/L).

Eukaryotic ModificationSecretableStable Expression
03

Insect System (Sf9)

Baculovirus-based, ideal for complex multi-subunit and membrane proteins, near-native conformation.

Complex ProteinsMembrane FriendlyHigh Activity
04

Mammalian Cells (HEK293 / CHO)

Closest to native conformation, suitable for therapeutic proteins, antibodies and VLPs.

Native ConformationPremium ApplicationsFull Modification

6-Step End-to-End Delivery

From gene to high-purity protein, every step strictly controlled.

01

Gene Synthesis

Codon-optimized high-expression gene synthesis.

02

Vector Construction

Sub-clone into expression vector with purification tag, sequence-verified.

03

Small-Scale Test

Test across expression systems for yield and solubility.

04

Scale-up Fermentation

Scale from 10mL to 50L with fine-tuned parameters.

05

Purification

Affinity + ion exchange + size exclusion, >90% purity.

06

QC & Delivery

SDS-PAGE / HPLC / endotoxin / activity assay with full QC report.

Multi-Gradient Fermenter Scales

10mL
Prokaryotic / Yeast / Insect / Mammalian
5-50mg
3-5 days
1L
All Systems Supported
100mg-1g
1-2 wks
10L
All Systems Supported
1-10g
2-3 wks
50L
All Systems Supported
10-50g
3-4 wks

Covering Core Life Science Fields

Structural Biology (Cryo-EM / X-ray)
Antibody Drug Development
IVD Materials
Enzyme Engineering
Vaccine R&D
Cell & Gene Therapy

Complete Delivery, Supporting Downstream Research

  • ✓High-purity protein (lyophilized / solution)
  • ✓Full QC report (purity / endotoxin / activity)
  • ✓Expression vector & sequence files
  • ✓Process documentation
  • ✓Long-term storage & re-supply

From mg to g Scale, No More Bottlenecks

50L fermenter + 4 expression systems + strict QC

Recombinant Protein Expression Service

From mg to 50L Fermenter Scale industrial protein

The Tenzobio recombinant protein expression platform, supplied exclusively through VONE BIO, integrates four expression systems (prokaryotic, yeast, insect, mammalian). Single-batch delivery up to 50L fermenter scale, high-purity protein for structural biology, antibody drug development and diagnostic materials.

Max Fermenter Scale50L
Protein Purity>90%
Endotoxin Level<1EU/mg
Success Rate90%

Four Expression Systems, Every Protein Covered

From prokaryotic to mammalian cells, from mg to gram scale, we find the optimal expression strategy for each protein.

01

Prokaryotic System (E.coli)

Cost-effective for enzymes, toxins, domains. Max yield up to 500mg/L.

Fast DeliveryHigh YieldCost-effective
02

Yeast System (Pichia)

Combines eukaryotic modification with yield, suitable for enzymes, cytokines (100-500mg/L).

Eukaryotic ModificationSecretableStable Expression
03

Insect System (Sf9)

Baculovirus-based, ideal for complex multi-subunit and membrane proteins, near-native conformation.

Complex ProteinsMembrane FriendlyHigh Activity
04

Mammalian Cells (HEK293 / CHO)

Closest to native conformation, suitable for therapeutic proteins, antibodies and VLPs.

Native ConformationPremium ApplicationsFull Modification

6-Step End-to-End Delivery

From gene to high-purity protein, every step strictly controlled.

01

Gene Synthesis

Codon-optimized high-expression gene synthesis.

02

Vector Construction

Sub-clone into expression vector with purification tag, sequence-verified.

03

Small-Scale Test

Test across expression systems for yield and solubility.

04

Scale-up Fermentation

Scale from 10mL to 50L with fine-tuned parameters.

05

Purification

Affinity + ion exchange + size exclusion, >90% purity.

06

QC & Delivery

SDS-PAGE / HPLC / endotoxin / activity assay with full QC report.

Multi-Gradient Fermenter Scales

10mL
Prokaryotic / Yeast / Insect / Mammalian
5-50mg
3-5 days
1L
All Systems Supported
100mg-1g
1-2 wks
10L
All Systems Supported
1-10g
2-3 wks
50L
All Systems Supported
10-50g
3-4 wks

Covering Core Life Science Fields

Structural Biology (Cryo-EM / X-ray)
Antibody Drug Development
IVD Materials
Enzyme Engineering
Vaccine R&D
Cell & Gene Therapy

Complete Delivery, Supporting Downstream Research

  • ✓High-purity protein (lyophilized / solution)
  • ✓Full QC report (purity / endotoxin / activity)
  • ✓Expression vector & sequence files
  • ✓Process documentation
  • ✓Long-term storage & re-supply

From mg to g Scale, No More Bottlenecks

50L fermenter + 4 expression systems + strict QC

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